Thin Layer Chromatography of lipids Assignment Example | Topics and Well Written Essays - 250 words

Thin Layer Chromatography of lipids - Assignment ExampleThe material allow dissolve in the solvent and run up the solvent front.(a) A Rf of 1 implies that the compound moved the corresponding distance as the hexane solvent. Therefore, the analyzed compound was highly non-polar because it travelled the aforesaid(prenominal) distance as hexane, which is a non-polar solvent.(b) Ethyl acetate is a polar solvent. Mixing it with hexane will reduce the hydrophobic nature of hexane (Nollet & Toldra, 2012). Consequently, the Rf value of the same compound will reduce since it will not fully dissolve in the mixture of hexane and ethyl acetate.The TLC system for phospholipase D reaction will include the enzyme itself, phosphatidylcholine and ethyl acetate as the polar solvent. Phospholipase D will hydrolyze the phosphatidylcholine to phosphatidic acid and choline. Choline is more polar compared to phosphatidic acid hence, it will move save than the acid. Molybdenum spray will be used to mon itor the movement of the separated material. At 2 minute, there is little hydrolysis, which then increases to the sixth minute. ensample phosphatidic will be used as a control in the